![]() The malonate test is useful in differentiating which members of enterobacteriaceae? Kligler iron agar (KIA) differs from triple sugar iron agar (TSI) in the Which genera are positive for phenlalanine deaminase? PAD Putrisine is an alkaline amine product of which bacterial enzyme? Lysine iron agar (LIA) showing a purple slant and a blackned butt indicates: decarboxylases split dibasic amino acids, forming alkaline amines. Which reagent is added to detect the production of indole.ĭecarboxylation of the amino acids lusine, ornathene and arginine results in the formation ofĪmines. In the test for urease production, ammonia reacts to form which product?Īmmonium carbonate. turns red to pink.Īt which Ph does the Methyl red test become positive.īlue color in the medium.alkaline. The volkes-proskauer (VP) test which end product of glucose fermentation.ĪCETOIN or carbinol. The ONPG detects B-galactosidase activity and is most useful in distinguishing late lactose fermenters from nonfermentesrs. The ortho-notriphenol-B- galactopyoanoside (ONPG) test is most useful when differentiting : ![]() Which class of antibiotics is used for the treatment of serious gram negative infections as well as infections with Mycoplasm tuberculosis.īiochemically the Enterobacteriaceae are gram negative rods that:įerment glugose, Reduce nitrate to nitrite, and are oxidase negative. Inoculun size, incubation time, Growth rate of bacteria.Īccording to the kirby bauer standard antimicrobial susceptibility testing method, what should be done when interpreting the zone size of a motle swarming organism such as proteus. Varibles that change the results of the MIC: The level of drug that is acheivable in serum. The breakpoint of an antimicrobial drug refers to: How to detect B-lactamase in bacteria Most sensitive. Incubated at 37 and cultured as soon as possible. Virus should be transported in media containing:Ĭerebrospinal fluid should be cultured immediately, but if delayed, specimen should be: ![]() Inoculate cyclohexide treated Mccoy cells. Prereduced vitamine K supplimented blood agar plates are recommended media forīacteroids, peptostreptococcus, and Clostridium spp. Hemophilus influenzia and Hemophilus para influenzia Xylose, lisine, Deoxycholateis a highly selective media for the recovery ofĮnterobacteriacae from gastrointestinal specimens.Ī sheep blood agar plate is used for the primary isolation medium when all of the following organisms are to be recovered from a wound EXCEPT: MacConkey and Colistin nalidixic acid (CNA) agars.Ĭharcoal transport, MTM media, New york or Martin Lewis. Primary and differential media of choice for the recovery of most fecal pathogens: Needle aspirate after surface decontamination Select the method of choice for recovery of bacteria from a deep abcess: Semisolid transport media such as amies, Stuart, or Cary Blair are suitable for the transport of swabs for culture of most pathogens except: The purpose of adding 0.025%-0.050% sodium polyanethol sulfonate (SPS) to the nutrient broth is:Ī flexable calcium alganite nasophrangeal swab is the collection device of choice for the recovery of which organism? When cleansing the skin with alcohol and then iodine for the collection of a blood culture, the iodine must stay on skin for:
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